New England, bidding to join fellow Major League Soccer side New York City in the remaining 4, were left shellshocked through a rampant Pumas at Mexico City's Estadio Olimpico.
A goal in each half from Pumas striker Juan Dinenno raised hopes of an improbable comeback earlier than Sebastian Saucedo lashed a strike into the roof of the net on fifty nine minutes to make it 3-0 on the night, three-3 on combination.
Both teams had chances to grab victory in a frenetic remaining passage of play, with New England's Omar Gonzalez seeing a header saved at point clean in stoppage time through Pumas goalkeeper Alfredo Talavera.
With no greater time, the sport went straight to consequences.
New England's Sebastian Lletget handed the initiative to Pumas after blazing his team's fourth penalty over the bar to go away the shoot-out at 2-2.
Nicolas Freire then scored to put Pumas three-2 ahead, and despite the fact that US global Jozy Altidore levelled along with his team's fifth kick, Dinenno stepped as much as fireplace the Mexican side into the semi-finals, prevailing the shoot-out four-three.
The defeat capped a disappointing night for MLS aspects within the tournament, the greatest club competition for groups from Central America, North America and the Caribbean (CONCACAF).
Earlier, Montreal had been held to a 1-1 draw at home to Liga-MX facet Cruz Azul, who went through to the semi-finals 2-1 on aggregate.
No crew from Major League Soccer has won the CONCACAF name when you consider that Los Angeles Galaxy lifted the crown in 2000.
